IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56724
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine
Cited:
Human, Mouse, Porcine
Applications
Validated:
Immunoprecipitation, Simple Western, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 39A1413
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa.
Modification
p Ser32, p Ser36
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - BSA Free
Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody.
Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lane 2) or without 1 nM TNF-a (lane 1). The membrane was blotted with NB100-56724 and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa.
Applications for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - BSA Free
Application
Recommended Usage
Immunoprecipitation
1-2 ug/ml
Simple Western
1:50
Western Blot
1-2 ug/ml
Application Notes
Simple Western reported by an internal validation: tested in MCF10A-/+EGF, Jurkat/-/+TNF, HEK293-/+UV; antibody dilution of 1:50; separated by charge
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IkB-alpha
Long Name
I-kappa-B-alpha
Alternate Names
IkBalpha, MAD-3, NFKBIA
Entrez Gene IDs
25493 (Rat)
Gene Symbol
NFKBIA
UniProt
Additional IkB-alpha Products
Product Documents for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - BSA Free
Product Specific Notices for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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