Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 39A1413

Format

Azide Free

Concentration

1.0 mg/ml

View all IkB-alpha Antibodies »

Product Specifications

Immunogen

This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa.

Modification

p Ser32, p Ser36

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free

Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free [NBP2-33247] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody. Image from the standard format of this antibody.
Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free [NBP2-33247] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lane 2) or without 1 nM TNF-a (lane 1). The membrane was blotted with NB100-56724 and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa. Image from the standard format of this antibody.

Applications for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free

Application
Recommended Usage

Immunoprecipitation

1-2 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS - BSA free

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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