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IKK beta Antibody (10AG2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56509

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10AG2

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Full-length recombinant human IKK beta protein (NP_001547).

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34333329)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IKK beta Antibody (10AG2) - BSA Free

Western Blot: IKK beta Antibody (10AG2)BSA Free [NB100-56509]

Western Blot: IKK beta Antibody (10AG2)BSA Free [NB100-56509]

Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Representative western blot of IKKB (E) in monocytes with quantification on the right. All data are representative of 5 separate experiments. Unpaired student t-test. ***p < 0.001. Image collected and cropped by CiteAb from the following publication (doi.org/10.3389/fimmu.2020.01478) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (10AG2) - BSA Free [NB100-56509]

Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (10AG2) - BSA Free [NB100-56509]

Immunocytochemistry/Immunofluorescence: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of IKK beta Antibody in HeLa cells. Antibody dilution 5 ug/ml. Image from verified customer review.
Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) - BSA Free [NB100-56509]

Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) - BSA Free [NB100-56509]

Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of IKK beta clone 10AG2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Applications for IKK beta Antibody (10AG2) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.25 - 0.5 ug/ 10^6 cells

Immunoblotting

reported in scientific literature (PMID 20103608)

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml. Use reported in scientific literature (PMID 24825920)

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500. Use reported in scientific literature (Page et al)

Immunoprecipitation

1:10 - 1:500

Simple Western

1:12.5

Western Blot

2 - 4 ug/ml
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-56509 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IKK beta

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Entrez Gene IDs

3551 (Human)

Gene Symbol

IKBKB

UniProt

Additional IKK beta Products

Product Documents for IKK beta Antibody (10AG2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IKK beta Antibody (10AG2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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