IKK beta Antibody (10AG2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80788
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 10AG2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
86.564 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IKK beta Antibody (10AG2) - Azide and BSA Free
Western Blot: IKK beta Antibody (10AG2)Azide and BSA Free [NBP2-80788]
Western Blot: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml. Image from the standard format of this antibody.Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788]
Immunocytochemistry/Immunofluorescence: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-IKK beta Antibody (10AG2) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788]
Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) - Azide and BSA Free [NBP2-80788] - Human gall bladder probed with IKKbeta antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT fApplications for IKK beta Antibody (10AG2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.25 - 0.5 ug/ 10^6 cells
Immunoblotting
reported in scientific literature (PMID 20103608)
Immunocytochemistry/ Immunofluorescence
2-5 ug/ml. Use reported in scientific literature (PMID 24825920)
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
reported in scientific literature (Page et al)
Immunoprecipitation
1:10 - 1:500
Simple Western
1:12.5
Western Blot
2 - 4 ug/ml
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IKK beta
Long Name
IkB Kinase beta
Alternate Names
IkBKB, IKK2, NFKBIKB
Gene Symbol
IKBKB
Additional IKK beta Products
Product Documents for IKK beta Antibody (10AG2) - Azide and BSA Free
Product Specific Notices for IKK beta Antibody (10AG2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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