IL-12 Antibody

Immunohistochemistry: IL-12 Antibody [NB600-1443]

Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro.IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] -

Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.HCa-1 & MIH-2 cells were injected intramuscularly into the right thighs of the mice, & tumors were irradiated with 10 Gy of radiation. Shown are IL-12 & DC (CD11c+) expressions at 1, 3, & 7 days after irradiation in (a) HCa-1 & (b) MIH-2 tumors & (c) IL-12 expression in the spleens of HCa-1 bearing mice (** P < 0.01). (d) IL-12 expression in BMDCs incubated with supernatant of MIH-2 tumor cells or supernatant of 10 Gy irradiating MIH-2 tumor cells. (** P < 0.01). DCs were differentiated from bone marrow of C3H/HeN normal mice (BMDCs). These were incubated for 72 h in supernatant of MIH-2 tumor cells with or without 10 Gy irradiation. IL-12 was intracellular stained with IL-12 antibody & analyzed by FACS. BMDCs also were stimulated with LPS (100 ng/mL) to allow maturation & irradiated with 10 Gy of radiation. (e) IL-12 expression (** P < 0.01), (f) cell viability, & (g) B7.1 expression in LPS stimulated BMDCs receiving 10 Gy radiation. (* P < 0.05). Data are from three independent experiments with five mice per group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] -

Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.IL-6 & IL-12 were measured at 1, 3, & 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 & IL-12 in (a) HCa-1 & (b) MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice & stimulated with LPS. (c) IL-6 expression analyzed at the indicated times by ELISA in irradiated or non-irradiated BMDCs in vitro. (d) IL-6R (** P < 0.01, vs No IR) & (e) sIL6R (** P < 0.01) were evaluated at 2 day after 10 Gy irradiation to BMDCs by FACS & ELISA, respectively. BMDCs were treated without or with IL-6 (20 ng/mL) plus LPS. (f) Down regulation of IL-12 production by IL-6 treatment in BMDCs (** P < 0.01). BMDCs were stimulated with LPS for activation & cultured with or without anti-IL-6 antibody (final concentration of 500 ng/mL) during the indicated times after irradiation in vitro. IL-6 & IL-12 were analyzed by ELISA. (g) Recovery of IL-12 production & (h) B7-1/ B7-2 expression using an anti-IL-6 antibody in irradiated-BMDCs stimulated with LPS in vitro. (** P < 0.01, * P < 0.05). Data are from three independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] -

Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.IL-6 & IL-12 were measured at 1, 3, & 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 & IL-12 in (a) HCa-1 & (b) MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice & stimulated with LPS. (c) IL-6 expression analyzed at the indicated times by ELISA in irradiated or non-irradiated BMDCs in vitro. (d) IL-6R (** P < 0.01, vs No IR) & (e) sIL6R (** P < 0.01) were evaluated at 2 day after 10 Gy irradiation to BMDCs by FACS & ELISA, respectively. BMDCs were treated without or with IL-6 (20 ng/mL) plus LPS. (f) Down regulation of IL-12 production by IL-6 treatment in BMDCs (** P < 0.01). BMDCs were stimulated with LPS for activation & cultured with or without anti-IL-6 antibody (final concentration of 500 ng/mL) during the indicated times after irradiation in vitro. IL-6 & IL-12 were analyzed by ELISA. (g) Recovery of IL-12 production & (h) B7-1/ B7-2 expression using an anti-IL-6 antibody in irradiated-BMDCs stimulated with LPS in vitro. (** P < 0.01, * P < 0.05). Data are from three independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] -

Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.HCa-1 & MIH-2 cells were injected intramuscularly into the right thighs of the mice, & tumors were irradiated with 10 Gy of radiation. Shown are IL-12 & DC (CD11c+) expressions at 1, 3, & 7 days after irradiation in (a) HCa-1 & (b) MIH-2 tumors & (c) IL-12 expression in the spleens of HCa-1 bearing mice (** P < 0.01). (d) IL-12 expression in BMDCs incubated with supernatant of MIH-2 tumor cells or supernatant of 10 Gy irradiating MIH-2 tumor cells. (** P < 0.01). DCs were differentiated from bone marrow of C3H/HeN normal mice (BMDCs). These were incubated for 72 h in supernatant of MIH-2 tumor cells with or without 10 Gy irradiation. IL-12 was intracellular stained with IL-12 antibody & analyzed by FACS. BMDCs also were stimulated with LPS (100 ng/mL) to allow maturation & irradiated with 10 Gy of radiation. (e) IL-12 expression (** P < 0.01), (f) cell viability, & (g) B7.1 expression in LPS stimulated BMDCs receiving 10 Gy radiation. (* P < 0.05). Data are from three independent experiments with five mice per group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.