IL-27 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16951

Novus Biologicals, part of Bio-Techne
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NBP2-16951
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IL-27 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL-27. The exact sequence is proprietary.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-27 Antibody

Western Blot: IL-27 Antibody [NBP2-16951]

Western Blot: IL-27 Antibody [NBP2-16951]

Western Blot: IL-27 Antibody [NBP2-16951] - Non-transfected (-) and IL27 -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IL27 antibody diluted by 1:1000.
Immunohistochemistry-Paraffin: IL-27 Antibody [NBP2-16951]

Immunohistochemistry-Paraffin: IL-27 Antibody [NBP2-16951]

Immunohistochemistry-Paraffin: IL27 Antibody [NBP2-16951] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

Applications for IL-27 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IL-27

The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq]

Long Name

Interleukin 27

Alternate Names

IL27

Gene Symbol

IL27

UniProt

Q8NEV9 (Human)

Additional IL-27 Products

Product Documents for IL-27 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-27 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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