IL-3 Antibody (OTI3B11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71023

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3B11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all IL-3 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 20-152 of human IL-3 (NP_000579) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-3 Antibody (OTI3B11) - Azide and BSA Free

Western Blot: IL-3 Antibody (OTI3B11)Azide and BSA Free [NBP2-71023]

Western Blot: IL-3 Antibody (OTI3B11)Azide and BSA Free [NBP2-71023]

Western Blot: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL3.
Immunocytochemistry/ Immunofluorescence: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023]

Immunocytochemistry/ Immunofluorescence: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023]

Immunocytochemistry/Immunofluorescence: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of A549 cells using anti-IL3 mouse monoclonal antibody.
Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023]

Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023]

Immunohistochemistry: IL-3 Antibody (OTI3B11) - Azide and BSA Free [NBP2-71023] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL3 mouse monoclonal antibody.
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Applications for IL-3 Antibody (OTI3B11) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-3

IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells. Recombinant human IL-3 is a 17 kDa globular protein consisting of 133 amino acid residues.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Gene Symbol

IL3

Additional IL-3 Products

Product Documents for IL-3 Antibody (OTI3B11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-3 Antibody (OTI3B11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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