Key Product Details

Species Reactivity

Human

Applications

Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6H6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IL-3R alpha Antibodies »

Product Specifications

Immunogen

Tissue / cell preparation. IL3R transfected COS cells.

Localization

Type I membrane protein.

Specificity

IL3RA (6H6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IL-3R alpha/CD123 Antibody (6H6) [DyLight 488]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [DyLight 488] [NB600-1185G] - Cell surface flow cytometric analysis using CD123 antibody. Human monocytes were probed using 10 ul of CD123 antibody (right) or 0.05 ug of isotype control was used for this test.

Applications for IL-3R alpha/CD123 Antibody (6H6) [DyLight 488]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-3R alpha

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Gene Symbol

IL3RA

Additional IL-3R alpha Products

Product Documents for IL-3R alpha/CD123 Antibody (6H6) [DyLight 488]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-3R alpha/CD123 Antibody (6H6) [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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