IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37354

Novus Biologicals, part of Bio-Techne
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NBP2-37354
NBP2-37354-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8E11C5

Format

BSA Free

Concentration

1 mg/ml

View all IL-3R alpha Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human IL-3R alpha/CD123 (AA: 200-305) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

Western Blot: IL-3R alpha/CD123 Antibody (8E11C5)BSA Free [NBP2-37354]

Western Blot: IL-3R alpha/CD123 Antibody (8E11C5)BSA Free [NBP2-37354]

Western Blot: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis using IL3RA mAb against HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free [NBP2-37354]

Immunocytochemistry/ Immunofluorescence: IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free [NBP2-37354]

Immunocytochemistry/Immunofluorescence: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis of Hela cells using IL3RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free [NBP2-37354]

Immunohistochemistry: IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free [NBP2-37354]

Immunohistochemistry: IL-3R alpha/CD123 Antibody (8E11C5) [NBP2-37354] - Analysis of lung cancer tissues using IL3RA mouse mAb with DAB staining.
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Applications for IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-3R alpha

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Gene Symbol

IL3RA

Additional IL-3R alpha Products

Product Documents for IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-3R alpha/CD123 Antibody (8E11C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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