IL-3R alpha/CD123 Antibody (IL3RA/2947R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79717

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # IL3RA/2947R

Concentration

0.2 mg/ml

View all IL-3R alpha Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human IL-3R alpha/CD123 protein (around aa 26-171) (exact sequence is proprietary) (Uniprot: P26951)

Localization

Cell Surface, Cytoplasm

Marker

Acute Myeloid Leukemia Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79851)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for IL-3R alpha/CD123 Antibody (IL3RA/2947R)

Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (IL3RA/2947R) [NBP2-79717]

Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (IL3RA/2947R) [NBP2-79717]

Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (IL3RA/2947R) [NBP2-79717] - Formalin-fixed, paraffin-embedded human Tonsil stained with IL-3R alpha/CD123 Antibody (IL3RA/2947R).
Protein Array: IL-3R alpha/CD123 Antibody (IL3RA/2947R) [NBP2-79717]

Protein Array: IL-3R alpha/CD123 Antibody (IL3RA/2947R) [NBP2-79717]

Protein Array: IL-3R alpha/CD123 Antibody (IL3RA/2947R) [NBP2-79717] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IL-3R alpha/CD123 Antibody (IL3RA/2947R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

Applications for IL-3R alpha/CD123 Antibody (IL3RA/2947R)

Application
Recommended Usage

Immunohistochemistry

0.25-0.5 ug/ml

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IL-3R alpha

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Gene Symbol

IL3RA

UniProt

P26951

Additional IL-3R alpha Products

Product Documents for IL-3R alpha/CD123 Antibody (IL3RA/2947R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-3R alpha/CD123 Antibody (IL3RA/2947R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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