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IL-4 Antibody (11B11) [Alexa Fluor® 532] - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52656AF532

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, In vivo assay

Label

Alexa Fluor 532

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 11B11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

BSF-1 / IL-4 purified from serum-free supernatants of cultured EL-4 cells (a C57BL/6 thymoma) induced with 4 Beta-phorbol 12 Beta-myristate 12 alpha -acetate for 48h.

Reactivity Notes

Mouse

Specificity

Binds to a core functional region of murine IL-4, which prevents this cytokine from interacting with its cognate receptor (Yao et al., 2006 PMID: 16361318).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for IL-4 Antibody (11B11) [Alexa Fluor® 532] - Chimeric

IL-4 Antibody (11B11) [Alexa Fluor® 532] - Chimeric

IL-4 Antibody (11B11) [Alexa Fluor® 532] - Chimeric [NBP2-52656AF532] -

IL-4 Antibody (11B11) [Alexa Fluor® 532] - Chimeric [NBP2-52656AF532] - Vial of Alexa Fluor 532 conjugated antibody. Alexa Fluor 532 is optimally excited at 532 nm by the Yellow-Green laser (561 nm) and has an emission maximum of 554 nm.

Applications for IL-4 Antibody (11B11) [Alexa Fluor® 532] - Chimeric

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

In vivo assay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-4

IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Gene Symbol

IL4

Additional IL-4 Products

Product Documents for IL-4 Antibody (11B11) [Alexa Fluor® 532] - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-4 Antibody (11B11) [Alexa Fluor® 532] - Chimeric



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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