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IL-7R alpha/CD127 Antibody (22F3D9) [Alexa Fluor® 405]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22376AF405

Novus Biologicals, part of Bio-Techne
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NBP2-22376AF405

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # 22F3D9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Amino acids 141-237 of human CD127 were used as the immunogen for the antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Scientific Data Images for IL-7R alpha/CD127 Antibody (22F3D9) [Alexa Fluor® 405]

Flow (Intracellular): IL-7 R alpha/CD127 Antibody (22F3D9) [Alexa Fluor (R) 405] [NBP2-22376AF405] - analysis of IL-7 R alpha in human PMBCs using anti-IL-7 R alpha antibody. Image from verified customer review.

Applications for IL-7R alpha/CD127 Antibody (22F3D9) [Alexa Fluor® 405]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-22376AF405 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-7R alpha/CD127

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Long Name

Interleukin 7 Receptor alpha

Alternate Names

CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha

Gene Symbol

IL7R

Additional IL-7R alpha/CD127 Products

Product Documents for IL-7R alpha/CD127 Antibody (22F3D9) [Alexa Fluor® 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-7R alpha/CD127 Antibody (22F3D9) [Alexa Fluor® 405]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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