IL-7R alpha/CD127 Antibody (22F3D9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80812
![Western Blot: IL-7R alpha/CD127 Antibody (22F3D9)Azide and BSA Free [NBP2-80812]](https://resources.bio-techne.com/images/products/IL-7R-alpha-CD127-Antibody-22F3D9-Azide-and-BSA-Free-Western-Blot-NBP2-80812-img0001.jpg "Western Blot: IL-7R alpha/CD127 Antibody (22F3D9)Azide and BSA Free [NBP2-80812]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Lambda Clone # 22F3D9
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Amino acids 141-237 of human CD127 were used as the immunogen for the antibody.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Lambda
Scientific Data Images for IL-7R alpha/CD127 Antibody (22F3D9) - Azide and BSA Free
Western Blot: IL-7R alpha/CD127 Antibody (22F3D9)Azide and BSA Free [NBP2-80812]
Western Blot: IL-7R alpha/CD127 Antibody (22F3D9) - Azide and BSA Free [NBP2-80812] - Analysis of CD127 in A) a partial recombinant protein and B) Daudi lysate using CD127 antibody at 0.5 ug/ml and 5 ug/ml respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.![Flow Cytometry: IL-7R alpha/CD127 Antibody (22F3D9) - Azide and BSA Free [NBP2-80812]](https://resources.bio-techne.com/images/products/IL-7R-alpha-CD127-Antibody-22F3D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80812-img0002.jpg "Flow Cytometry: IL-7R alpha/CD127 Antibody (22F3D9) - Azide and BSA Free [NBP2-80812]")
Flow Cytometry: IL-7R alpha/CD127 Antibody (22F3D9) - Azide and BSA Free [NBP2-80812]
Flow Cytometry: IL-7R alpha/CD127 Antibody (22F3D9) - Azide and BSA Free [NBP2-80812] - Analysis of IL-7 R alpha in human PMBCs using anti-IL-7 R alpha antibody. Image from verified customer review. Image using the Alexa Fluor 405 format of this antibody. Image from the standard format of this antibody.Applications for IL-7R alpha/CD127 Antibody (22F3D9) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100. Use reported by customer review
Western Blot
2-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. The amino acid sequence used as immunogen for the antibody is present in two isoforms of CD127: 459 amino acids and 299 amino acids. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-7R alpha/CD127
Long Name
Interleukin 7 Receptor alpha
Alternate Names
CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha
Gene Symbol
IL7R
Additional IL-7R alpha/CD127 Products
Product Documents for IL-7R alpha/CD127 Antibody (22F3D9) - Azide and BSA Free
Product Specific Notices for IL-7R alpha/CD127 Antibody (22F3D9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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