ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42998

Clone HP-F1 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-42998-0.025mg
NBP1-42998-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HP-F1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all LILRB1/CD85j/ILT2 Antibodies »

Product Summary for ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free

Immunogen

The immunogen for this antibody was CD85j.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free

Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free [NBP1-42998]

Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free [NBP1-42998]

Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) [NBP1-42998] - Analysis using the Biotin conjugate of NBP1-42998. Staining of normal human peripheral blood cells with 0.125 micrograms of Mouse IgG1 k Isotype Control Biotin (blue histogram) or Anti-Human CD85j (ILT2) Biotin (purple histogram) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free [NBP1-42998]

Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free [NBP1-42998]

Flow Cytometry: ILT2/CD85j/LILRB1 Antibody (HP-F1) [NBP1-42998] - Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (blue histogram) or Anti-Human CD85j (ILT2) APC (purple histogram). Cells in the monocyte gate were used for analysis.

Applications for ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000
Application Notes
This low endotoxin and azide free HP-F1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 uL (0.25 ug) per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells per test.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Endotoxin Level

< 0.001 ng/ug.

Formulation

PBS (pH 7.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LILRB1/CD85j/ILT2

The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T and B cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F. The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1

Alternate Names

CD85j, ILT2, LIR1, MIR7

Gene Symbol

LILRB1

Additional LILRB1/CD85j/ILT2 Products

Product Documents for ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ILT2/CD85j/LILRB1 Antibody (HP-F1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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