ILT2/CD85j/LILRB1 Antibody (PSH04-59)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32537
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH04-59
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human ILT2/CD85j/LILRB1 aa 1-461 / 650. (Uniprot: Q8NHL6)
Localization
Cell membrane; Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ILT2/CD85j/LILRB1 Antibody (PSH04-59)
Western Blot: ILT2/CD85j/LILRB1 Antibody (PSH04-59) [NBP3-32537]
Western Blot: ILT2/CD85j/LILRB1 Antibody (PSH04-59) [NBP3-32537] - Western blot analysis of ILT2/CD85j/LILRB1 on different lysates with Rabbit anti-ILT2/CD85j/LILRB1 antibody (NBP3-32537) at 1/1,000 dilution.Lane 1: Raji cell lysate
Lane 2: Daudi cell lysate
Lane 3: THP-1 cell lysate
Lane 4: A431 cell lysate (negative)
Lane 5: K-562 cell lysate (negative)
Lysates/proteins at 20 ug/Lane.
Predicted band size: 71 kDa
Observed band size: 120 kDa
Exposure time: 1 minute; ECL;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32537) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for ILT2/CD85j/LILRB1 Antibody (PSH04-59)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LILRB1/CD85j/ILT2
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1
Alternate Names
CD85j, ILT2, LIR1, MIR7
Gene Symbol
LILRB1
Additional LILRB1/CD85j/ILT2 Products
Product Documents for ILT2/CD85j/LILRB1 Antibody (PSH04-59)
Product Specific Notices for ILT2/CD85j/LILRB1 Antibody (PSH04-59)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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