Integrin alpha L/CD11a Antibody (MEM-25) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB110-81625
Clone MEM-25 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunoprecipitation
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-25
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Leukocytes from a patient suffering from a LGL-type leukemia (NM_001114380.1).
Specificity
The antibody MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. HLDA IV; WS Code NL 209
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications for Integrin alpha L/CD11a Antibody (MEM-25) [Biotin]
Application
Recommended Usage
Flow Cytometry
2-3 ug/ml
Immunoprecipitation
1:50
Application Notes
Flow Cytometry: use at an assay-dependent dilution Immunoprecipitation: use at an assay-dependent dilutions (suitable for immunoaffinity purification of LFA-1 complex). Functional Applications: use at an assay-dependent dilution (blocks binding of LFA-1 complex to ICAM-1) Other applications have not been tested. Optimal dilutions should be determined by end-user. Purity is > 95% (by SDS-PAGE).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Integrin alpha L/CD11a
Alternate Names
CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21
Gene Symbol
ITGAL
Additional Integrin alpha L/CD11a Products
Product Documents for Integrin alpha L/CD11a Antibody (MEM-25) [Biotin]
Product Specific Notices for Integrin alpha L/CD11a Antibody (MEM-25) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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