Integrin alpha L/CD11a Antibody (MEM-25) - Low Endotoxin, Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37712

Clone MEM-25 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Block/Neutralize, CyTOF-ready, Flow Cytometry, Functional, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-25

Format

Low Endotoxin, Azide and BSA Free

Concentration

1 mg/ml

View all Integrin alpha L/CD11a Antibodies »

Product Specifications

Immunogen

Leukocytes from a patient suffering from a LGL-type leukemia (NM_001114380.1).

Specificity

The antibody MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. HLDA IV; WS Code NL 209

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) - Low Endotoxin, Azide and BSA Free [NBP2-37712]

Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) - Low Endotoxin, Azide and BSA Free [NBP2-37712]

Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) - Low Endotoxin and Azide Free [NBP2-37712] - Separation of human monocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11a (MEM-25) purified antibody (concentration in sample 1 ug/ml) GAM APC.
Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) - Low Endotoxin, Azide and BSA Free [NBP2-37712]

Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) - Low Endotoxin, Azide and BSA Free [NBP2-37712]

Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) - Low Endotoxin and Azide Free [NBP2-37712] - Analysis using the PE conjugate of NB500-328. Surface staining of human peripheral blood cells with anti-human CD11a (MEM-25) PE.
Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) - Low Endotoxin, Azide and BSA Free [NBP2-37712]

Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) - Low Endotoxin, Azide and BSA Free [NBP2-37712]

Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) - Low Endotoxin and Azide Free [NBP2-37712] - Staining pattern of human peripheral whole blood stained using anti-human CD11a (MEM-25) purified antibody (concentration in sample 1 ug/ml) GAM APC.

Applications

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunoprecipitation

1:10-1:500Blocking/Neutralizing 50-200 molar excess
Application Notes
Immunoprecipitation: excellent antibody for immunoaffinity purification of LFA-1 complex
Blocking/Neutralizing: this antibody partially blocks binding of LFA-1 complex to ICAM-1

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 0.2 um filter sterilized

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha L/CD11a

CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.

Alternate Names

CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21

Gene Symbol

ITGAL

Additional Integrin alpha L/CD11a Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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