Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # IDH1/1152

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Isocitrate Dehydrogenase 1/IDH1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Isocitrate Dehydrogenase 1/IDH1 protein (around aa 281-414) (exact sequence is proprietary) (Uniprot: O75874 )

Localization

Cytoplasmic & Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Isocitrate Dehydrogenase 1/IDH1 Mouse Monoclonal Antibody (Isocitrate Dehydrogenase 1/IDH1/1152).
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302] - Human Colon Carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302] - Human Prostate Carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).

Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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