Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70988CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP1-47804

Alexa Fluor 350

NBP2-70988AF350

Alexa Fluor 405

NBP2-70988AF405

Alexa Fluor 488

NBP2-70988AF488

Alexa Fluor 532

NBP2-70988AF532

Alexa Fluor 594

NBP2-70988AF594

Alexa Fluor 647

NBP2-70988AF647

Alexa Fluor 700

NBP2-70988AF700

Alexa Fluor 750

NBP2-70988AF750

Allophycocyanin

NBP2-70988APC

Biotin

NBP2-70988B

DyLight 350

NBP2-70988UV

DyLight 405

NBP2-70988V

DyLight 488

NBP2-70988G

DyLight 550

NBP2-70988R

DyLight 594

NBP2-70988DL594

DyLight 650

NBP2-70988C

DyLight 680

NBP2-70988FR

DyLight 755

NBP2-70988IR

FITC

NBP2-70988F

HRP

NBP2-70988H

Janelia Fluor 525

NBP2-70988JF525

Janelia Fluor 549

NBP2-70988JF549

Janelia Fluor 585

NBP2-70988JF585

Janelia Fluor 635

NBP2-70988JF635

Janelia Fluor 646

NBP2-70988JF646

Janelia Fluor 669

NBP2-70988JF669

PE

NBP2-70988PE

PerCP

NBP2-70988PCP

Forumulation

Catalog #

Azide and BSA Free

NBP2-70988

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI2H9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Isocitrate Dehydrogenase 1/IDH1 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human IDH1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [CoraFluor™ 1]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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