Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Isocitrate Dehydrogenase 1/IDH1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 364 to 414 of human Isocitrate Dehydrogenase 1 using the numbering given in entry NP_005887.2 (GeneID 3417).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Isocitrate Dehydrogenase 1/IDH1 Antibody

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-32150]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-32150]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-32150] - Samples: Whole cell lysate (50 ug) from K562, HeLa, 293T, mouse TCMK-1, mouse NIH3T3, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Isocitrate dehydrogenase antibody NBP2-32150 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-32150]

Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-32150]

Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-32150] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Isocitrate dehydrogenase antibody NBP2-32150 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Isocitrate dehydrogenase, NBP2-32150 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:500 - 1:2500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

UniProt

NP_005887.2

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents for Isocitrate Dehydrogenase 1/IDH1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.