KAT3B/p300 Antibody (RW105) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-616
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat, Mustelid, Primate
Cited:
Human, Mouse
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Electron Microscopy, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, Knockdown Validated, Western Blot
Cited:
Chemotaxis, Electron Microscopy, Immunoprecipitation, In-vitro, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # RW105
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
Reactivity Notes
Mink.
Localization
Nuclear
Specificity
This is specific for p300 protein. This recognizes residues 1921-2023.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for KAT3B/p300 Antibody (RW105) - BSA Free
Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) - BSA Free [NB100-616]
Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW105)) at 5ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.Western Blot: KAT3B/p300 Antibody (RW105)BSA Free [NB100-616]
Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) - BSA Free [NB100-616]
Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B/p300 Antibody (RW105) NB100-616 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.Applications for KAT3B/p300 Antibody (RW105) - BSA Free
Application
Recommended Usage
Electron Microscopy
reported in scientific literature (PMID 25728767)
Flow (Intracellular)
1ug/mL
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunoprecipitation
1:10-1:500
In vitro assay
reported in scientific literature (PMID 25728767)
Knockdown Validated
Validated for Knockdown from a verified customer review.
Western Blot
1:250-1:500
Reviewed Applications
Read 1 review rated 5 using NB100-616 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: p300
Long Name
E1A-binding Protein p300
Alternate Names
EP300
Gene Symbol
EP300
Additional p300 Products
Product Documents for KAT3B/p300 Antibody (RW105) - BSA Free
Product Specific Notices for KAT3B/p300 Antibody (RW105) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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