Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Mustelid, Primate

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Electron Microscopy, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, Knockdown Validated, Western Blot

Cited:

Chemotaxis, Electron Microscopy, Immunoprecipitation, In-vitro, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RW105

Format

BSA Free

Concentration

1 mg/ml

View all p300 Antibodies »

Product Specifications

Immunogen

Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]

Reactivity Notes

Mink.

Localization

Nuclear

Specificity

This is specific for p300 protein. This recognizes residues 1921-2023.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for KAT3B/p300 Antibody (RW105) - BSA Free

Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) - BSA Free [NB100-616]

Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) - BSA Free [NB100-616]

Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW105)) at 5ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.
Western Blot: KAT3B/p300 Antibody (RW105)BSA Free [NB100-616]

Western Blot: KAT3B/p300 Antibody (RW105)BSA Free [NB100-616]

Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.
Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) - BSA Free [NB100-616]

Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) - BSA Free [NB100-616]

Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B/p300 Antibody (RW105) NB100-616 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
View 4 more images

Applications for KAT3B/p300 Antibody (RW105) - BSA Free

Application
Recommended Usage

Electron Microscopy

reported in scientific literature (PMID 25728767)

Flow (Intracellular)

1ug/mL

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:10-1:500

In vitro assay

reported in scientific literature (PMID 25728767)

Knockdown Validated

Validated for Knockdown from a verified customer review.

Western Blot

1:250-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-616 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p300

In the eukaryotic genome, chromatin dynamicity is crucial for regulation of processes requiring access to genetic information and is regulated by several different factors including post-translational modifications of histone/non-histone components, especially via acetylation process which is catalyzed by enzymes known as lysine acetyltransferases (KATs). Among different KATs, KAT3B/ p300 and its homologue KAT3A/CBP (CREB-binding protein) are extensively studied KATs with well known cellular functions. KAT3B/ p300 and KAT3A/CBP have been shown to directly interact with several different DNA-binding transcription factors which includes nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Moreover, both KAT3B/p300 and KAT3A/CBP have been shown to exert HAT (histone acetyltransferase) activity, capable of acetylating all four core histone particles in nucleosomes and to play a key role in activating chromatin for transcription. KAT3B/p300 is a potent transcriptional coactivator that has been shown to regulate various biological processes such as cellular proliferation, differentiation, cell cycle, apoptosis, and response to genetic damage, and KAT3B/ p300 dysfunction interfering with these processes implicates in the pathogenesis of several diseases such as cancer, cardiac diseases, diabetes mellitus, and HIV.

Long Name

E1A-binding Protein p300

Alternate Names

EP300

Gene Symbol

EP300

Additional p300 Products

Product Documents for KAT3B/p300 Antibody (RW105) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KAT3B/p300 Antibody (RW105) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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