KAT3B/p300 Antibody (RW105) [DyLight 650]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-616C
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Mustelid, Primate
Applications
Chromatin Immunoprecipitation (ChIP), Electron Microscopy, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, Knockdown Validated, Western Blot
Label
DyLight 650 (Excitation = 652 nm, Emission = 672 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # RW105
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
Reactivity Notes
Human, mouse, rat, monkey, and mink.
Localization
Nuclear
Specificity
This is specific for p300 protein. This recognizes residues 1921-2023.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for KAT3B/p300 Antibody (RW105) [DyLight 650]
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
Electron Microscopy
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
In vitro assay
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This KAT3B/p300 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot. In Western blot a band is seen at ~300 kDa, representing the p300 protein. Lower non-specific MW bands (~45-66 kDa) ay be seen with longer exposure times. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: p300
Long Name
E1A-binding Protein p300
Alternate Names
EP300
Gene Symbol
EP300
Additional p300 Products
Product Documents for KAT3B/p300 Antibody (RW105) [DyLight 650]
Product Specific Notices for KAT3B/p300 Antibody (RW105) [DyLight 650]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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