KAT3B/p300 Antibody (RW128) - BSA Free

Western Blot: KAT3B/p300 Antibody (RW128)BSA Free [NB100-507]

Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Kinase active Src is present in the nuclei of Panc-1 cells in association with p300. Subcellular fractionation and localization of Src and p300 in Panc-1 cells. Cell lysates of equal total protein or Panc-1 subcellular fractions were subjected to immunoblotting analysis for Src, pSrc, p300, tubulin (cytoskeletal proteins), GAPDH (soluble cytoplasmic proteins), calnexin (endoplasmic reticulum), grim19 (mitochondria), histone H3 (chromatin), and lamin A/C (nuclear lamina). Image collected and cropped by Citeab from the following publication (A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget (2016) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW128) - BSA Free [NB100-507]

Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B/p300 Antibody (RW128) NB100-507 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: KAT3B/p300 Antibody (RW128) - BSA Free [NB100-507]

Flow Cytometry: KAT3B/p300 Antibody (RW128) [NB100-507] - An intracellular stain was performed on Raw264.7 cells with KAT3B/p300 Antibody (RW128) NB100-507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Western Blot: KAT3B/p300 Antibody (RW128)BSA Free [NB100-507]

Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Kinase active Src is present in the nuclei of Panc-1 cells in association with p300. Src and p300 co-association in Panc-1 cells. Immunoblotting analysis of Src or p300 immunecomplexes from Panc-1 nuclear isolates probing for Src or p300, or IgG control. Image collected and cropped by Citeab from the following publication (A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget (2016) licensed under a CC-BY license.