Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Mustelid, Primate

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RW128

Format

Azide and BSA Free

Concentration

1 mg/ml

View all p300 Antibodies »

Product Summary for KAT3B/p300 Antibody (RW128) - Azide and BSA Free

Immunogen

Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]

Epitope

The epitope recognized is found between amino acids 1868-1921.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

300 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KAT3B/p300 Antibody (RW128) - Azide and BSA Free

Western Blot: KAT3B/p300 Antibody (RW128)Azide and BSA Free [NBP2-80819]

Western Blot: KAT3B/p300 Antibody (RW128)Azide and BSA Free [NBP2-80819]

Western Blot: KAT3B/p300 Antibody (RW128) - Azide and BSA Free [NBP2-80819] - Detection of p300 in a HeLa nuclear extract using NB100-507 (1:250). ECL: 15 sec exposure. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW128) - Azide and BSA Free [NBP2-80819]

Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW128) - Azide and BSA Free [NBP2-80819]

Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) - Azide and BSA Free [NBP2-80819] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW128) at 5 ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective. Image from the standard format of this antibody.
Flow Cytometry: KAT3B/p300 Antibody (RW128) - Azide and BSA Free [NBP2-80819]

Flow Cytometry: KAT3B/p300 Antibody (RW128) - Azide and BSA Free [NBP2-80819]

Flow Cytometry: KAT3B/p300 Antibody (RW128) - Azide and BSA Free [NBP2-80819] - An intracellular stain was performed on Raw264.7 cells with KAT3B/p300 Antibody (RW128) NBP2-80819 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).
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Applications for KAT3B/p300 Antibody (RW128) - Azide and BSA Free

Application
Recommended Usage

Flow (Intracellular)

1 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10 - 1:2000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:250 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p300

In eukaryotic genome, the chromatin dynamicity is crucial for regulation of processes requiring access to genetic information and it is regulated by several different factors including post-translational modifications of histone/non-histone components, especially via acetylation process which is catalyzed by enzymes known as lysine acetyltransferases (KATs). Among different KATs, KAT3B/ p300 and its homologue KAT3A/CBP (CREB-binding protein) are extensively studied KATs with well known cellular functions. KAT3B/ p300 and KAT3A/CBP have been shown to directly interact with several different DNA-binding transcription factors which includes nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Moreover, both KAT3B/p300 and KAT3A/CBP have been shown to exert HAT (histone acetyltransferase) activity, capable of acetylating all four core histone particles in nucleosomes and to play a key role in activating chromatin for transcription. KAT3B/p300 is a potent transcriptional coactivator that has been shown to regulate various biological processes such as cellular proliferation, differentiation, cell cycle, apoptosis, and response to genetic damage, and KAT3B/ p300 dysfunction interfering with these processes implicates in the pathogenesis of several diseases such as cancer, cardiac diseases, diabetes mellitus, and HIV.

Long Name

E1A-binding Protein p300

Alternate Names

EP300

Gene Symbol

EP300

Additional p300 Products

Product Documents for KAT3B/p300 Antibody (RW128) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KAT3B/p300 Antibody (RW128) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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