Lamin B1 Antibody (CL3929)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59783

Novus Biologicals, part of Bio-Techne
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NBP2-59783-25ul
NBP2-59783
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3929

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lamin B1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RTTRGKRKRVDVEESEASSSVSISHSASA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Lamin B1 Antibody (CL3929)

Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783]

Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783]

Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783] - Analysis in human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of A-431 cells showing specific staining of nuclear membrane in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human colon shows nuclear membrane positivity in glandular and connective tissue cells.
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Applications for Lamin B1 Antibody (CL3929)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-59783 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin B1

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Alternate Names

ADLD, LMN2, LMNB1

Gene Symbol

LMNB1

Additional Lamin B1 Products

Product Documents for Lamin B1 Antibody (CL3929)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lamin B1 Antibody (CL3929)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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