Lamin B1 Antibody (CL3929) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44038

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3929

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Lamin B1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P20700, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Lamin B1 Antibody (CL3929) - Azide and BSA Free

Lamin B1 Antibody (CL3929) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody [NBP3-44038]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody [NBP3-44038]

Staining of A-431 cells using the Anti-Lamin B1 monoclonal antibody) .
Lamin B1 Antibody (CL3929) - Azide and BSA Free Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP3-44038]

Immunohistochemistry-Paraffin: Lamin B1 Antibody [NBP3-44038]

Staining of human cerebellum shows strong nuclear membrane positivity in cells in granular layer.
Lamin B1 Antibody (CL3929) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody [NBP3-44038]

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody [NBP3-44038]

Staining of HeLa cells using the Anti-Lamin B1 monoclonal antibody) .
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Applications for Lamin B1 Antibody (CL3929) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin B1

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Alternate Names

ADLD, LMN2, LMNB1

Gene Symbol

LMNB1

Additional Lamin B1 Products

Product Documents for Lamin B1 Antibody (CL3929) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lamin B1 Antibody (CL3929) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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