LAT [p Tyr191] Antibody (2E4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15162
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2E4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Phospho-LAT (Y191). UniProt ID (O43561).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LAT [p Tyr191] Antibody (2E4)
Western Blot: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate (treated with EGF or Pervanadate). All lanes: LAT antibody at 2.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa.
Immunocytochemistry/Immunofluorescence: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - Immunofluorescence staining of Hela cells with at LAT antibody 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - IHC image of LAT antibody diluted at 1:100 and staining in paraffin-embedded human colon cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Applications for LAT [p Tyr191] Antibody (2E4)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: LAT
Long Name
Linker for Activation of T cells
Alternate Names
pp36
Gene Symbol
LAT
Additional LAT Products
Product Documents for LAT [p Tyr191] Antibody (2E4)
Product Specific Notices for LAT [p Tyr191] Antibody (2E4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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