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LAT Antibody (SR1593)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22544

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-22544-100ul
NBP3-22544-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1593

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for LAT Antibody (SR1593)

Immunogen

A synthesized peptide derived from human LAT (Uniprot #: O43561)

Specificity

Detects endogenous levels of total LAT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LAT Antibody (SR1593)

Western Blot: LAT Antibody (SR1593) [NBP3-22544] -

Western Blot: LAT Antibody (SR1593) [NBP3-22544] - Western blot analysis using NBP3-22544 in Jurkat cell lysate.

Applications for LAT Antibody (SR1593)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAT

LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Long Name

Linker for Activation of T cells

Alternate Names

pp36

Gene Symbol

LAT

Additional LAT Products

Product Documents for LAT Antibody (SR1593)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LAT Antibody (SR1593)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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