LAT [p Tyr220] Antibody (SR1644)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21508

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21508-100ul
NBP3-21508-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1644

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LAT Antibodies »

Product Summary for LAT [p Tyr220] Antibody (SR1644)

Immunogen

A synthesized peptide derived from human phospho-LAT [Tyr220] (Uniprot #: O43561)

Modification

p Tyr 220

Specificity

Detects endogenous levels of total phospho-LAT [Tyr220]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LAT [p Tyr220] Antibody (SR1644)

Western Blot: LAT [p Tyr 220] Antibody (SR1644) [NBP3-21508] -

Western Blot: LAT [p Tyr 220] Antibody (SR1644) [NBP3-21508] - Western blot analysis using NBP3-21508 in Jurkat cell treated with CD3 lysate.

Applications for LAT [p Tyr220] Antibody (SR1644)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:30

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAT

LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Long Name

Linker for Activation of T cells

Alternate Names

pp36

Gene Symbol

LAT

Additional LAT Products

Product Documents for LAT [p Tyr220] Antibody (SR1644)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LAT [p Tyr220] Antibody (SR1644)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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