LAT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1441

Novus Biologicals, part of Bio-Techne
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NB100-1441
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LAT Antibodies »

Product Summary for LAT Antibody

Immunogen

Peptide with sequence C-GAPDYENLQELN corresponding to C-Terminus according to NP_055202.1, NP_001014987.1, NP_001014989.1, NP_001014988.1.

Specificity

This antibody is expected to recognize isoform a (NP_055202.1), isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LAT Antibody

Western Blot: LAT Antibody [NB100-1441]

Western Blot: LAT Antibody [NB100-1441]

Western Blot: LAT Antibody [NB100-1441] - (0.01ug/ml) staining of peripheral blood mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for LAT Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in human lymph node and peripheral Brood mononucleocytes lysates and in mouse and rat thymus lysates (calculated MW of 27.9 kDa band according to NP_055202.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LAT

Linker for activation of T cells (LAT) is an integral membrane protein (36 to 38 kD), which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. LAT antibody stains thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and spleen. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells.

Long Name

Linker for Activation of T cells

Alternate Names

pp36

Entrez Gene IDs

27040 (Human)

Gene Symbol

LAT

UniProt

NP_055202. (Human)

Additional LAT Products

Product Documents for LAT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LAT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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