Key Product Details

Species Reactivity

Mouse

Applications

Agonist Activity, CyTOF-ready, Flow Cytometry, Immunocytochemistry

Label

CoraFluor 1

Antibody Source

Monoclonal Rat IgG2B Clone # 803420

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Lgr5/GPR49 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Lgr5/GPR49
Lys375-Pro547
Accession # Q9Z1P4

Specificity

Detects mouse Lgr5/GPR49 in ELISA. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD20", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Lgr5/GPR49 Antibody (803420) [CoraFluor™ 1]

Application
Recommended Usage

Agonist Activity

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Lgr5/GPR49

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Gene Symbol

LGR5

Additional Lgr5/GPR49 Products

Product Documents for Lgr5/GPR49 Antibody (803420) [CoraFluor™ 1]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lgr5/GPR49 Antibody (803420) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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