Detects mouse Lgr5/GPR49 in ELISA. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD20", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse Lgr5/GPR49 Antibody

Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse Lgr5/GPR49 and eGFP was stained with and either (A) Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgf5/GPR49.

Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Lgr5/GPR49 in Mouse Cells Transfected with Mouse Lgr5/GPR49.

Lgr5/GPR49 was detected in immersion fixed MYC tagged mouse transfectants using Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240). Cells were stained using an anti-Mouse HRP-conjugated secondary antibody (brown).Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands.

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Applications for Mouse Lgr5/GPR49 Antibody

Application

Recommended Usage

Agonist Activity

Peng, W.C., et al. (2013) Cell Reports. 3:1885.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse Lgr5/GPR49 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with GFP tagged mouse LGR5

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr5/GPR49

Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the mouse Lgr5 long extracellular domain used as an immunogen shares 90% and 95% amino acid sequence identity with human and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5⁺ stem cells can produce all epithelial cell types of the intestinal crypts (4). It is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD20" by Peng et al.(1).

References

Peng et al. (2013) Cell Rep. 3(6):1885.
de Lau et al. (2014) Genes Dev. 28(4):305.
Barker et al. (2013) Development 140(12):2484.
Clevers (2013) Nature 495(7439):53.
Wu et al. (2014) Nat Commun 5:3149.
Jang et al. (2013) PLoS One 8(12):e82390495.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Entrez Gene IDs

8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)

Gene Symbol

LGR5

UniProt

Q9Z1P4

Additional Lgr5/GPR49 Products

Product Documents for Mouse Lgr5/GPR49 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Lgr5/GPR49 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Lgr5/GPR49 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8240

Key Product Details

Species Reactivity

Validated:

Cited:

Applications