LXR alpha/NR1H3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB400-157

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Primate

Cited:

Human, Mouse, Porcine

Predicted:

Chicken (90%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.04 mg/ml

View all LXR alpha/NR1H3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human LXR alpha/NR1H3 protein sequence (between residues 50-150). [UniProt# Q13133]

Reactivity Notes

Immunogen sequence has 92% identity to rat and 90% identity to chicken.

Localization

Nuclear (potential)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LXR alpha/NR1H3 Antibody

Western Blot: LXR alpha/NR1H3 Antibody [NB400-157]

Western Blot: LXR alpha/NR1H3 Antibody [NB400-157]

Western Blot: LXR alpha/NR1H3 Antibody [NB400-157] - WB detection of LXR in human liver.
Simple Western: LXR alpha/NR1H3 Antibody [NB400-157]

Simple Western: LXR alpha/NR1H3 Antibody [NB400-157]

Simple Western: LXR alpha/NR1H3 Antibody [NB400-157] - Simple Western lane view shows a ~49 kDa for LXR in human skeletal muscle tissue lysate under reducing conditions. Image from verified customer review.

Applications for LXR alpha/NR1H3 Antibody

Application
Recommended Usage

Western Blot

0.5-2 ug/ml
Application Notes
This LXR alpha/NR1H3 antibody is useful for Western blot, where a band is observed at ~50 kDa.

Reviewed Applications

Read 1 review rated 3 using NB400-157 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1.04 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LXR alpha/NR1H3

LXR (liver X receptors - LXR-alpha and LXR-beta, encoded by NR1H3 and NR1H2, respectively) belongs to nuclear hormone receptor superfamily and are ligand-dependent nuclear transcription factors which are activated by oxysterols. LXR beta is ubiquitously expressed whereas LXR alpha is expressed mainly in liver, intestine, adipose tissue and macrophages. LXR-alpha and LXR-beta shares 78% similarity within their ligand-binding domains and form obligate heterodimers with retinoic X receptor (RXR), bind specific response elements within promoters of target genes ABCA1, ABCG1, SREBF1, APOE etc., and regulate their expression. In the unliganded state, LXRs binds with corepressors such as N-CoR which displaces upon ligand binding. Oxysterols (oxidized cholesterol derivatives) are their natural ligands whereas endogenous ligands includes 22(R)-hydroxycholesterol (22-HC), 24(S)-hydroxycholesterol (24(S)-HC), 24,25-EC , 27-hydroxycholesterol etc. LXRs control the major metabolic pathways such as cholesterol homeostasis, lipogenesis, inflammation, toxicity proliferation cell survival/death and different aspacts of acquired/innate immunity.

Long Name

Liver X Receptor alpha

Alternate Names

NR1H3, RLD-1

Gene Symbol

NR1H3

Additional LXR alpha/NR1H3 Products

Product Documents for LXR alpha/NR1H3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LXR alpha/NR1H3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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