Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82107

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Lysine (K)-specific Demethylase 6B/KDM6B Antibodies »

Product Summary for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3. The immunogen is located within amino acids 1370-1420 of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3. Amino Acid Sequence: KVPGSRTPGHQENNNF

Specificity

This antibody is specific for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 and will not recognize other JMJD proteins. Human Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 has 2 isoforms, including isoform 1 (1682aa, 181kD) and isoform 2 (1643aa, 177kD). Mouse Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 has one isoform (1641aa, 176kD) and rat Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 also has one isoform (1356aa, 146kD). NBP2-82107 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in HepG2 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 ug/mL Lane 2: 0.5 ug/mL Lane 3: 0.25 ug/mL with blocking peptide
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP2-82107]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP2-82107]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Immunofluorescence Validation of JMJD3 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 with NBP2-82107 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in Human, Mouse, and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 [NBP2-82107], (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Applications for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry

2.5 ug/mL

Immunocytochemistry/ Immunofluorescence

2.5-20 ug/mL

Western Blot

0.25-4 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 6B/KDM6B

JMJD3 is a histone demethylase that specifically demethylates trimethylated and dimethylated 'Lys-27' of histone H3, thereby playing a central role in histone code. JMJD3 is also important for the regulation of posterior development by regulating HOX gene expression. JMJD3 is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. It has also been shown to be essential to mammalian epidermal differentiation and is upregulated in prostate cancer.

JMJD3 antibodies are useful tools for epigenetics research and specifically for histone modification studies.

Alternate Names

JMJD3, KDM6B, Lysine (K)specific Demethylase 6B

Gene Symbol

KDM6B

Additional Lysine (K)-specific Demethylase 6B/KDM6B Products

Product Documents for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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