Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82107CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
CoraFluor 1
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3. The immunogen is located within amino acids 1370-1420 of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3. Amino Acid Sequence: KVPGSRTPGHQENNNF
Specificity
This antibody is specific for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 and will not recognize other JMJD proteins. Human Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 has 2 isoforms, including isoform 1 (1682aa, 181kD) and isoform 2 (1643aa, 177kD). Mouse Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 has one isoform (1641aa, 176kD) and rat Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 also has one isoform (1356aa, 146kD). NBP2-82107 can detect human, mouse and rat.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Lysine (K)-specific Demethylase 6B/KDM6B
JMJD3 antibodies are useful tools for epigenetics research and specifically for histone modification studies.
Alternate Names
JMJD3, KDM6B, Lysine (K)specific Demethylase 6B
Gene Symbol
KDM6B
Additional Lysine (K)-specific Demethylase 6B/KDM6B Products
Product Specific Notices
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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