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Lysozyme Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2005

Novus Biologicals, part of Bio-Techne
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NB100-2005-0.002g

Key Product Details

Species Reactivity

Validated:

Chicken

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Lysozyme Antibody [Biotin]

Immunogen

This purified Lysozyme Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme. (Uniprot: Q6LEL2)

Reactivity Notes

A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined

Specificity

This purified antibody is directed against lysozyme from hen egg white protein.

Marker

Histiocytic Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

This antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White]

Scientific Data Images for Lysozyme Antibody [Biotin]

Western Blot: Lysozyme Antibody [Biotin] [NB100-2005] - Lane 1:Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Predicted/Observed size: 4.9kDa, 5 kDa for Lysozyme. Other band(s): none.

Applications for Lysozyme Antibody [Biotin]

Application
Recommended Usage

ELISA

1:20000

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:100

Western Blot

1:2000-1:10000
Application Notes
This product has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysozyme

Lysozyme catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N acetylmuramic acid and N acetylglucosamine. It is found in spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears.

Alternate Names

EC 3.2.1.17, lysozyme, lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C, lysozyme C, LZM, renal amyloidosis

Gene Symbol

LYZ

Additional Lysozyme Products

Product Documents for Lysozyme Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lysozyme Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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