Lysozyme Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-850
Key Product Details
Species Reactivity
Chicken
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This purified Lysozyme Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme. (Uniprot: Q6LEL2)
Reactivity Notes
A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined
Specificity
This purified antibody is directed against lysozyme from hen egg white protein.
Marker
Histiocytic Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White]
This antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White]
Scientific Data Images for Lysozyme Antibody [HRP]
Western Blot: Lysozyme Antibody [HRP] [NB600-850] - Lane 1:Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: incubated with blocking buffer for Lysozyme. Other band(s): none.
Applications for Lysozyme Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000
Immunohistochemistry
1:500 - 1:2500
Immunohistochemistry-Paraffin
1:500 - 1:2500
Immunoprecipitation
1:100
Western Blot
1:1000 - 1:5000
Application Notes
This product has been tested by western blotting and ELISA and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Lysozyme
Alternate Names
EC 3.2.1.17, lysozyme, lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C, lysozyme C, LZM, renal amyloidosis
Gene Symbol
LYZ
UniProt
Additional Lysozyme Products
Product Specific Notices for Lysozyme Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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