MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25556

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25556
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2118

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAP3K8/Tpl2/COT Antibodies »

Product Summary for MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free

Immunogen

Recombinant fragmemt of human MAP3K8/Tpl2/COT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free

MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free

Western Blot: MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free [NBP3-25556] -

Western Blot: MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free [NBP3-25556] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [HL2118] (NBP3-25556) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free

Western Blot: MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free [NBP3-25556] -

Western Blot: MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free [NBP3-25556] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [HL2118] (NBP3-25556) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free

Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free [NBP3-25556] -

Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free [NBP3-25556] - MAP3K8 antibody [HL2118] detects MAP3K8 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. MAP3K8 stained by MAP3K8 antibody [HL2118] (NBP3-25556) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP3K8/Tpl2/COT

The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 8

Alternate Names

COT, EST, ESTF, MAP3K8, Tpl-2

Gene Symbol

MAP3K8

Additional MAP3K8/Tpl2/COT Products

Product Documents for MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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