Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3D9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MAP3K8/Tpl2/COT Antibodies »

Product Summary for MAP3K8/Tpl2/COT Antibody (OTI3D9) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 277-461 of human MAP3K8(NP_005195) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAP3K8/Tpl2/COT Antibody (OTI3D9) - Azide and BSA Free

Western Blot: MAP3K8/Tpl2/COT Antibody (OTI3D9)Azide and BSA Free [NBP2-72589]

Western Blot: MAP3K8/Tpl2/COT Antibody (OTI3D9)Azide and BSA Free [NBP2-72589]

Western Blot: MAP3K8/Tpl2/COT Antibody (OTI3D9) - Azide and BSA Free [NBP2-72589] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K8.

Applications for MAP3K8/Tpl2/COT Antibody (OTI3D9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAP3K8/Tpl2/COT

The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 8

Alternate Names

COT, EST, ESTF, MAP3K8, Tpl-2

Gene Symbol

MAP3K8

Additional MAP3K8/Tpl2/COT Products

Product Documents for MAP3K8/Tpl2/COT Antibody (OTI3D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MAP3K8/Tpl2/COT Antibody (OTI3D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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