MBD3 Antibody (PCRP-MBD3-1C4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08535

Novus Biologicals, part of Bio-Techne
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NBP3-08535
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-MBD3-1C4

Format

Azide and BSA Free

Concentration

1 mg/ml

View all MBD3 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human MBD3 protein (Uniprot: O95983)

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07604).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MBD3 Antibody (PCRP-MBD3-1C4) - Azide and BSA Free

Flow Cytometry: MBD3 Antibody (PCRP-MBD3-1C4) - Azide and BSA Free [NBP3-08535]

Flow Cytometry: MBD3 Antibody (PCRP-MBD3-1C4) - Azide and BSA Free [NBP3-08535]

Flow Cytometry: MBD3 Antibody (PCRP-MBD3-1C4) [NBP3-08535] - Flow Cytometric Analysis of PFA-fixed HeLa cells. MBD3 Mouse Monoclonal Antibody (PCRP-MBD3-1C4) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: MBD3 Antibody (PCRP-MBD3-1C4) - Azide and BSA Free [NBP3-08535]

Protein Array: MBD3 Antibody (PCRP-MBD3-1C4) - Azide and BSA Free [NBP3-08535]

Protein Array: MBD3 Antibody (PCRP-MBD3-1C4) [NBP3-08535] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MBD3 Mouse Monoclonal Antibody (PCRP-MBD3-1C4).

Applications for MBD3 Antibody (PCRP-MBD3-1C4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MBD3

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).

Long Name

Methyl-CpG Binding Domain Protein 3

Alternate Names

methyl-CpG binding domain protein 3, methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3

Gene Symbol

MBD3

Additional MBD3 Products

Product Documents for MBD3 Antibody (PCRP-MBD3-1C4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MBD3 Antibody (PCRP-MBD3-1C4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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