DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).

Long Name

Methyl-CpG Binding Domain Protein 3

Alternate Names

methyl-CpG binding domain protein 3, methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3

Gene Symbol

MBD3

Additional MBD3 Products

Product Documents for MBD3 Antibody (PCRP-MBD3-1C4) [Alexa Fluor® 700]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for MBD3 Antibody (PCRP-MBD3-1C4) [Alexa Fluor® 700]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Spectra Viewer
Flow Cytometry

MBD3 Antibody (PCRP-MBD3-1C4) [Alexa Fluor® 700]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08535AF700

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all MBD3 Antibodies »

Product Specifications

Immunogen

Localization

Clonality

Host

Isotype

Applications for MBD3 Antibody (PCRP-MBD3-1C4) [Alexa Fluor® 700]

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Protein Array

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: MBD3