Mcl-1 Antibody (8C6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2194
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 8C6
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human MCL-1 expressed in E. Coli.
Localization
Cell Membrane, Cytoplasmic, Nuclear
Specificity
MCL-1 (8C6D4B1)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Mcl-1 Antibody (8C6) - BSA Free
![Western Blot: Mcl-1 Antibody (8C6)BSA Free [NB100-2194]](https://resources.bio-techne.com/images/products/Mcl-1-Antibody-8C6D4B1-Western-Blot-NB100-2194-img0008.jpg "Western Blot: Mcl-1 Antibody (8C6)BSA Free [NB100-2194]")
Western Blot: Mcl-1 Antibody (8C6)BSA Free [NB100-2194]
Western Blot: Mcl-1 Antibody (8C6D4B1) [NB100-2194] - Analysis using MCL1 mouse mAb against Hela (1), BCBL-1 (2), Jurkat (3) and HL60 (4) cell lysate.![Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]](https://resources.bio-techne.com/images/products/Mcl-1-Antibody-8C6D4B1-Immunocytochemistry-Immunofluorescence-NB100-2194-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]")
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]
Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (8C6D4B1) [NB100-2194] - Analysis of HepG2 cells using MCL1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.![Immunohistochemistry-Paraffin: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]](https://resources.bio-techne.com/images/products/Mcl-1-Antibody-8C6D4B1-Immunohistochemistry-Paraffin-NB100-2194-img0007.jpg "Immunohistochemistry-Paraffin: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]")
Immunohistochemistry-Paraffin: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]
Immunohistochemistry-Paraffin: Mcl-1 Antibody (8C6D4B1) [NB100-2194] - Analysis of human lymphnode tissues using MCL1 mouse mAb with DAB staining.Applications for Mcl-1 Antibody (8C6) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500-1:2000
Application Notes
This antibody is useful for ELISA, Western Blo, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Mcl-1
Long Name
Myeloid Cell Leukemia Sequence 1
Alternate Names
BCL2L3, Mcl1
Entrez Gene IDs
4170 (Human)
Gene Symbol
MCL1
Additional Mcl-1 Products
Product Documents for Mcl-1 Antibody (8C6) - BSA Free
Product Specific Notices for Mcl-1 Antibody (8C6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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