Mcl-1 Antibody (OTI10F6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72598

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI10F6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Mcl-1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mcl-1 Antibody (OTI10F6) - Azide and BSA Free

Western Blot: Mcl-1 Antibody (OTI10F6)Azide and BSA Free [NBP2-72598]

Western Blot: Mcl-1 Antibody (OTI10F6)Azide and BSA Free [NBP2-72598]

Western Blot: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598]

Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598]

Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MCL-1.
Flow Cytometry: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598]

Flow Cytometry: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598]

Flow Cytometry: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal and then analyzed by flow cytometry.
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Applications for Mcl-1 Antibody (OTI10F6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:200 - 1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mcl-1

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Long Name

Myeloid Cell Leukemia Sequence 1

Alternate Names

BCL2L3, Mcl1

Gene Symbol

MCL1

Additional Mcl-1 Products

Product Documents for Mcl-1 Antibody (OTI10F6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mcl-1 Antibody (OTI10F6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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