Mcl-1 Antibody (S04-3A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19546

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-3A4

Concentration

0.3 mg/ml

View all Mcl-1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Mcl-1 (Uniprot # Q07820)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mcl-1 Antibody (S04-3A4)

Western Blot: Mcl-1 Antibody (S04-3A4) [NBP3-19546]

Western Blot: Mcl-1 Antibody (S04-3A4) [NBP3-19546]

Western Blot: Mcl-1 Antibody (S04-3A4) [NBP3-19546] - Western blot detection of Mcl-1 in C6 cell lysates using NBP3-19546 (1:1000 diluted). Predicted band size: 37kDa. Observed band size: 40kDa.
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (S04-3A4) [NBP3-19546]

Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (S04-3A4) [NBP3-19546]

Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (S04-3A4) [NBP3-19546] - Immunofluorescence of Mcl-1 (green) in MCF-7 using NBP3-19546 at dilution 1/20, and DAPI (blue)

Applications for Mcl-1 Antibody (S04-3A4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mcl-1

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Long Name

Myeloid Cell Leukemia Sequence 1

Alternate Names

BCL2L3, Mcl1

Gene Symbol

MCL1

Additional Mcl-1 Products

Product Documents for Mcl-1 Antibody (S04-3A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mcl-1 Antibody (S04-3A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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