MeCP2 [p Ser80] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47558

Novus Biologicals, part of Bio-Techne
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NBP1-47558
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all MeCP2 Antibodies »

Product Summary for MeCP2 [p Ser80] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser80 conjugated to KLH. Accession # P51608

Modification

p Ser80

Specificity

Specific for the ~75 kDa MECP2 protein phosphorylated at Ser80 in Western blots of human, rat and mouse brain extracts. The antibody has also been used successfully for immunohistochemistry on mouse brain sections.

Marker

Nucleus Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for MeCP2 [p Ser80] Antibody

Western Blot: MeCP2 [p Ser80] Antibody - Nucleus Marker [NBP1-47558] - Western blot analysis of MecP2 on neonatal rat brain using NBP1-47558.

Applications for MeCP2 [p Ser80] Antibody

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:100 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Serum

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MeCP2

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).

Alternate Names

AUTSX3, DKFZp686A24160, MeCp-2 protein, mental retardation, X-linked 79, methyl CpG binding protein 2 (Rett syndrome), X-linked 16

Entrez Gene IDs

4204 (Human)

Gene Symbol

MECP2

UniProt

P51608 (Human)

Additional MeCP2 Products

Product Documents for MeCP2 [p Ser80] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MeCP2 [p Ser80] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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