MeCP2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81027
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (98%), Rat (99%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESS
Reactivity Notes
Reactivity reported in scientific literature (PMID: 23435261)
Marker
Nucleus Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MeCP2 Antibody
Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027]
Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human cerebellum, lymph node, prostate and testis using Anti-MeCP2 antibody NBP1-81027 (A) shows similar protein distribution across tissues to independent antibody NBP1-81028 (B).Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody [NBP1-81027]
Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP1-81027] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027]
Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.Applications for MeCP2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MeCP2
Alternate Names
AUTSX3, DKFZp686A24160, MeCp-2 protein, mental retardation, X-linked 79, methyl CpG binding protein 2 (Rett syndrome), X-linked 16
Entrez Gene IDs
4204 (Human)
Gene Symbol
MECP2
UniProt
Additional MeCP2 Products
Product Documents for MeCP2 Antibody
Product Specific Notices for MeCP2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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