MeCP2 [p Ser421] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23407

Novus Biologicals, part of Bio-Techne
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NBP3-23407
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MeCP2 Antibodies »

Product Specifications

Immunogen

phospho-MeCP2 (Ser-421) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 421 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins. [UniProt# Q9Z2D6]

Modification

p Ser421

Specificity

The antibody detects a 75 kDa protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for MeCP2 [p Ser421] Antibody

MeCP2 [p Ser421] Antibody

Western Blot: MeCP2 [p Ser421] Antibody [NBP3-23407] -

Western Blot: MeCP2 [p Ser421] Antibody [NBP3-23407] - Western blot of human PC3 cells treated with calyculin A (25 mM) for 15 min. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (C-terminus) (lanes 1 & 2) or anti-MeCP2 (Ser-421) (lanes 3 & 4).
MeCP2 [p Ser421] Antibody

Immunocytochemistry/Immunofluorescence: MeCP2 [p Ser421] Antibody [NBP3-23407] -

Immunocytochemistry/Immunofluorescence: MeCP2 [p Ser421] Antibody [NBP3-23407] - Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (Ser-421) rabbit polyclonal antibody (NBP3-23407) in the absence (left) or presence (right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.

Applications for MeCP2 [p Ser421] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: MeCP2

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Alternate Names

AUTSX3, DKFZp686A24160, MeCp-2 protein, mental retardation, X-linked 79, methyl CpG binding protein 2 (Rett syndrome), X-linked 16

Gene Symbol

MECP2

Additional MeCP2 Products

Product Documents for MeCP2 [p Ser421] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MeCP2 [p Ser421] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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