Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34341

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa/IgG1 Kappa Clone # DT101+ BC199 + HMB45

Concentration

0.2 mg/ml

View all Melanoma Marker (MART-1 + gp100) Antibodies »

Product Specifications

Immunogen

Recombinant Melanoma Marker (MART-1 + gp100) protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Localization

Cytoplasmic

Specificity

This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa/IgG1 Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34608)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341]

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341]

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45).
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341]

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341]

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (DT101+ BC199 + HMB45).

Applications for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Flow Cytometry reported in scientific literature (PMID: 30735560).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Melanoma Marker (MART-1 + gp100)

Alternate Names

MART1, MART-1

Gene Symbol

MLANA

UniProt

Q16655, P40967 (Human)

Additional Melanoma Marker (MART-1 + gp100) Products

Product Documents for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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