Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34608
![Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - Azide and BSA Free [NBP2-34608]](https://resources.bio-techne.com/images/products/Melanoma-Marker-MART-1-+-gp100-Antibody-DT101+-BC199-+-HMB45-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34608-img0001.jpg "Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - Azide and BSA Free [NBP2-34608]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa/IgG1 Kappa Clone # DT101+ BC199 + HMB45
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant Melanoma Marker (MART-1 + gp100) protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Localization
Cytoplasmic
Specificity
This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa/IgG1 Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34341).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - Azide and BSA Free [NBP2-34608]
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - Azide and BSA Free [NBP2-34608] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45).![Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - Azide and BSA Free [NBP2-34608]](https://resources.bio-techne.com/images/products/Melanoma-Marker-MART-1-+-gp100-Antibody-DT101+-BC199-+-HMB45-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34608-img0002.jpg "Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - Azide and BSA Free [NBP2-34608]")
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - Azide and BSA Free [NBP2-34608]
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - Azide and BSA Free [NBP2-34608] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (DT101+ BC199 + HMB45).Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Flow Cytometry reported in scientific literature (PMID: 30735560).
Optimal dilution for a specific application should be determined.
Use in Flow Cytometry reported in scientific literature (PMID: 30735560).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Melanoma Marker (MART-1 + gp100)
Alternate Names
MART1, MART-1
Gene Symbol
MLANA
Additional Melanoma Marker (MART-1 + gp100) Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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