Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34547
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow (Intracellular), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Kappa/IgG2a Kappa/IgG1 Kappa Clone # A103 + T311 + HMB45
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary
Immunogen
Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (A103); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Localization
Cytoplasmic
Specificity
This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Kappa/IgG2a Kappa/IgG1 Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34339).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images
Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45)Azide and BSA Free [NBP2-34547]
Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - Azide and BSA Free [NBP2-34547] - Western Blot Analysis of COLO-38 cell lysate using Melanoma Marker (MART-1 + Tyrosinase + gp100) MAb (A103 + T311 + HMB45).Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - Azide and BSA Free [NBP2-34547]
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - Azide and BSA Free [NBP2-34547] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (A103 + T311 + HMB45).Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5-1.0ug/ml
Western Blot
2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Melanoma Marker (MART-1 + Tyrosinase + gp100)
Alternate Names
MART1, MART-1, melan-A, MLANA
Gene Symbol
MLANA
Additional Melanoma Marker (MART-1 + Tyrosinase + gp100) Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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